The correct option is B Closing and opening of stomata
Transpiration is the process of loss of water, in vapour form, through stomata. The process depends on the turgidity of guard cell. An increase in turgidity of guard cells opens stomatal pore, whereas a decrease in turgidity of guard cells closes stomatal pore. The change in turgidity of guard cells is brought about by reversible influx and efflux of potassium ions.