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B
Protoplast fusion
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C
Embryoid culture
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D
Pollen culture
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Solution
The correct option is D Pollen culture Pollen culture is a technique of production of haploid plants by the culture of pollen grains obtained from an anther of a plant under the aseptic condition on artificial media of known composition. The technique was discovered by Guha and Maheshwari. The pollen's were obtained by squeezing the anthers after surface sterilizing with hypochlorite. The obtained pollen's were cultured on nutrient media.