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B
Production of homozygous diploids
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C
Microdensitometer studies
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D
Induction of giant cells
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Solution
The correct option is A Production of callus Chromosome counts showed that the callus produced was haploid when the anthers 1) were of a dark-brown to black color, and 2) were cultured in the late tetrad stage of microspore development.. Diploid plants were obtained when the anthers had been cultured in the dark.