Hardening in tissue culture is

Keeping at 30-50C temperature for about 30 minutes

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Acclimatisation of tissue culture plants slowly before growing in the field

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Plunging the vials into water at 37-40C

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None of the above

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Solution

The correct option is B Acclimatisation of tissue culture plants slowly before growing in the field
The acclimatisation of plant formed by tissue culture before growing in the field to make it strong to adapt in new environment.

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