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Question

Holoenzyme is the complete enzyme consisting of an apoenzyme and a cofactor. Select the option that correctly identifies the nature of apoenzyme and cofactor.


A

Apoenzyme - Protein, Cofactor - Non-protein

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B

Apoenzyme - Non-protein, Cofactor - Protein

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C

Apoenzyme - Protein, Cofactor - Protein

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D

Apoenzyme - Non-protein, Cofactor - Non-protein

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Solution

The correct option is A

Apoenzyme - Protein, Cofactor - Non-protein


Enzymes may be broadly classified into two types depending on their chemical composition simple enzymes and conjugated enzymes. Simple enzymes are wholly made up of proteins and any additional substance or group is absent, e.g., pepsin, trypsin etc.

Conjugated enzymes (or holoenzymes) are formed of two parts – a protein part called apoenzyme and a non-protein part named co-factor. The complete conjugated enzyme consisting of an apoenzyme and a co-factor is called holoenzyme. Holoenzyme is the functional unit of enzyme.

Holo enzyme(active enzyme)Apo enzyme(protein part)+Co - factor(nonprotein part)

Co-factor may be inorganic or organic in nature. Catalytic activity is lost when co-factor is removed from the enzyme which indicates that it plays a crucial role in the catalytic activity of enzymes.


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