The correct option is A Auxin and Cytokinin
In plant tissue culture, the concentration of growth regulators in the medium must be optimized and may change according to the desired result. For example, auxin is often the primary phytohormone to support callus formation and maintenance. Cytokinins promote shoot formation, but need auxins as well to promote mitosis. So, specific auxin to cytokinin ratios in plant tissue culture medium give rise to an unorganized, growing and dividing mass of callus and also leads to callus differentiation.