Question

How can you differentiate genomic library and cDNA library?

Answer 1

• A genomic library is prepared from the total DNA of a cell line or tissue, whereas acDNA library represents the population of mRNAs in a tissue.
• Genomic DNA libraries are prepared by performing partial digestion of total DNA with a restriction enzyme that cuts DNA frequently. On the other hand, cDNA libraries are prepared by first isolating all the mRNAs in a tissue and then copying these molecules into double-stranded DNA, using the enzymes reverse transcriptase and DNA polymerase.