Question

How do restriction sites work?

Answer 1

Restriction sites:

1. Restriction sites have a unique 4-8 nucleotide sequence that restriction enzymes recognize.
2. Restriction enzymes are enzymes that cut DNA at or near the restriction site.

Restriction enzymes:

1. A restriction enzyme is a bacterial protein that cleaves DNA sequences at sequence-specific places, resulting in DNA fragments having a known sequence at each end.
2. Certain laboratory processes, such as recombinant DNA technology and genetic engineering, require the use of restriction enzymes.