Question

How do you Identify a Peptide Bond?

Answer 1

To identify the peptide bond:

• A peptide bond is formed by the interaction between the carboxyl group of one molecule and the amino group of other molecules by releasing water. The reaction between these two molecules leads to the formation of peptide bonds and a new molecule is called amide. These bonds are covalent bonds.
• The Biuret test is a test which is used for the identification of peptide bonds in a molecule. The ${\mathrm{Cu}}^{2+}$ ion forms a complex with the amide group presence in the molecule. The indication of violet colour shows the presence of a peptide bond.

Thus, by the Biuret test we can identify the peptide bond in a molecule.