How does a restriction nucleases function? Explain.

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Solution

Restriction endonuclease, is the most widely used enzyme for the process of gene modification. It recognizes a specific sequence of nucleotides which is known as recognition site and produces a double strand nick in the respective DNA. The action of restriction endonuclease can be on palindromic sequences. For example, EcoRI cuts the DNA at the following palindromic sequence:
5' GAATTC 3'

3'CTT AAG 5'

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