Question

How does stirring the broth in the bioreactor help?

• A. Avoids contamination
• B. Causes the cells to declump
• C. Improves aeration among cells
• D. Decreases frothing

Answer 1

Answer: (B), (C)

The correct options are
B Causes the cells to declump
C Improves aeration among cells

When the broth is allowed to settle in the bioreactor, the cells will eventually fall to the bottom and start to clump. Clumping of the cells will lead to lesser availability of oxygen and nutrient for growth. This is not an ideal situation. Stirring helps in removing any clump formation and promotes better aeration.