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B
By specialized bacteria in their leaves
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C
Independent of bacteria that live in them
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D
Not by any of the above methods
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Solution
The correct option is A By specialized bacteria in their roots Nitrogen fixation is the conversion of atmospheric nitrogen into nitrates and ammonia by nitrogen-fixing bacteria which are associated with the roots of the legume plants.
Examples of nitrogen fixing bacteria includes Rhizobium
So, the correct answer is 'By specialized bacteria in their roots'