Question

How human insulin is produced from biotechnology? (3 marks/5 marks)

Answer 1

Production of Genetic Engineered Insulin
1. Human insulin is extracted from pancreas cells and an insulin-producing gene is isolated.
2. A plasmid DNA is extracted from a bacterium and cut with restriction enzyme, forming plasmid vector.
3. Insert human insulin-producing gene into the bacterial plasmid vector to form the recombinant DNA of human insulin-producing gene.
4. Introduce this recombinant DNA into a bacterial cell to form the recombinant bacterium.
5. The recombinant bacteria multiply in a fermentation tank and produce human insulin.
6. Insulin is extracted, purified and bottled. It is then ready to be injected into diabetic patients..