Question

How is the coding sequence of the lac Z gene considered a better selectable marker?

Answer 1

Coding sequence of the lac Z gene

1. Lac Z gene also called β - galactosidase
2. The gene of the enzyme lac Z is another unique marker.
3. When an external gene is inserted into the lac Z gene, the enzyme lac Z gene ceases to function.
4. Bacteria are then planted in a chromogenic substrate.
5. Non-recombinants will produce blue colonies, while recombinants will produce colorless colonies.