CameraIcon
CameraIcon
SearchIcon
MyQuestionIcon
MyQuestionIcon
1
You visited us 1 times! Enjoying our articles? Unlock Full Access!
Question

How is the amplification of gene sample of interest carried out using polymerase chain reaction (PCR) ?

Open in App
Solution

The polymerase chain reaction is used to reproduce selected sections of DNA or RNA for analysis. Previously, amplification of DNA involved cloning the segments of interest into vectors for expression in bacteria.
The three steps are used for amplification
1. Denaturing - When the double-stranded template DNA is heated to separate it into two single strands.
2.Annealing - When the temperature is lowered to enable the DNA primers to attach to the template DNA
3.Extension - Polymerisation of nucleotide chain by the enzyme. Taq polymerase using the nucleotides provided in the medium.

flag
Suggest Corrections
thumbs-up
0
Join BYJU'S Learning Program
similar_icon
Related Videos
thumbnail
lock
Biotechnology and Its Application
BIOLOGY
Watch in App
Join BYJU'S Learning Program
CrossIcon