Question

How is the outcome of PCR evaluated?

Answer 1

PCR:

1. It is a method for quickly and correctly producing many copies of a certain DNA fragment.
2. As with every DNA synthesis reaction, PCR is based on three straightforward steps: (1) denaturing the template into single strands; (2) annealing primers to each original strand for new strand synthesis; and (3) extending the new DNA strands from the primers.
3. Gel electrophoresis is typically used to view the outcomes of a PCR process.
4. DNA fragments are separated based on size using the process of gel electrophoresis, which involves moving DNA fragments through a gel matrix with an electric current.