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Question

How is the retention factor or Rf of a particular compound measured in a Thin layer of Chromatography (TLC) experiment?

A
Distance traveled by the compound on the TLC plate divided by the total distance traveled by the mobile phase
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B
Distance traveled by the compound on the TLC plate multiplied by the total distance traveled by the mobile phase
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C
The total distance traveled by the mobile phase divided by distance traveled by the compound on the TLC plate
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D
The total distance traveled by the mobile phase multiplied by distance traveled by the compound on the TLC plate
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Solution

The correct option is A Distance traveled by the compound on the TLC plate divided by the total distance traveled by the mobile phase
Option A, is the correct answer. In general, the compound whose structure is similar to the mobile phase in the TLC, will have a high retention factor. The compound whose structure is similar to the stationery phase on the TLC, will have a low retention factor.

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