The correct option is A 1
Statement IV is incorrect.
Hungarian engineer Karl Ereky first coined the term 'biotechnology' in 1919, meaning the production of products from raw materials with the aid of living organisms.
Statements I, II and III are correct.
The enzyme DNA ligase is used to join DNA fragments. Ligases are enzymes that are capable of catalysing the reaction of joining two large molecules by establishing a new chemical bond. For example, DNA ligase catalyses the joining of the 3′-OH to the 5′-phosphate, by forming a phosphodiester bond between two successive nucleotides.
The plasmid used to create the first recombinant DNA was isolated from Escherichia coli. Stanley Cohen and Herbert Boyer generated the first recombinant DNA molecule in 1972, by combining an antibiotic resistant gene from a bacterium with plasmid of Escherichia coli.
Asexual reproduction preserves the genetic information, while sexual reproduction permits variation. Sexual reproduction provides opportunities for variations and formulation of unique combinations of genetic setup, some of which may be beneficial to the organism as well as the population.