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Question

How many protons are accumulated in intermembrane space of mitochondria from one molecule of NADH during ETS?

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Solution

Electrons accumulated in the intermembrane space

In complex I, the oxidation of NADH releases electrons which is transported to ubiquinone which is present in the inner membrane.

During this process, for each pair of electron being transported to ubiquinone from NADH through FMN and FeS center, complex I pumps four protons into the inner membrane space.

Final answer:

(C) Four

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