Question

How to purify DNA from cell extract?

Answer 1

Deoxyribonucleic acid:

1. The molecule found inside cells that carries the genetic material necessary for an organism to develop and function properly is referred to as DNA. This knowledge can be passed on from one generation to the next due to DNA molecules.
2. RNA and proteins are present in sufficient amounts in bacterial cell extract.
3. Such a combination of DNA can be purified using a variety of techniques.
4. One approach involves treating the mixture with chemicals that break down the impurities and leave behind a pure DNA solution using enzyme digestion and organic extraction to remove pollutants.
5. Ion-exchange chromatography is an alternative method for decomposing a mixture into its constituent parts.
6. As a result, the extract's RNA and proteins are devoid of DNA.