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Question
Identify A, B, C and D in the given figure of E. coli cloning vector pBR322 and select the correct option.
Identify A, B, C and D in the given figure of E. coli cloning vector pBR322 and select the correct option.
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Solution
The correct option is D A - EcoR I, B - BamH I, C - ampR, D - ori
pBR322 is a plasmid-based cloning vector which was developed by Bolivar and Rodriguez (BR in the name stands for the names of the developers). Plasmid vectors are used for cloning small DNA fragments.
Following is the identification of labels along with the characteristic features of a plasmid:
Recognition sequences of various common restriction enzymes are inserted in the plasmid for introducing a variety of genes of interest.
Here, in the image, Pvu I, Pst I, Cla I, Hind III are a few restriction sites. The labels, A and B are also coding for two other restriction sites of the restriction enzymes, EcoRI and BamHI, respectively.
Antibiotic resistance genes are inserted to differentiate between transformed cells and non-transformed cells.
Transformed cells are those cells which have taken up the plasmid DNA. When the cells are grown on antibiotic-containing medium, only transformed cells will survive.
In the image, C, which is ampR along with tetR are the antibiotic resistance genes.
Here, in the image, D is "ori" or origin of replication. This is the region from where replication is initiated.
pBR322 is a plasmid-based cloning vector which was developed by Bolivar and Rodriguez (BR in the name stands for the names of the developers). Plasmid vectors are used for cloning small DNA fragments.
Following is the identification of labels along with the characteristic features of a plasmid:
Recognition sequences of various common restriction enzymes are inserted in the plasmid for introducing a variety of genes of interest.
Here, in the image, Pvu I, Pst I, Cla I, Hind III are a few restriction sites. The labels, A and B are also coding for two other restriction sites of the restriction enzymes, EcoRI and BamHI, respectively.
Antibiotic resistance genes are inserted to differentiate between transformed cells and non-transformed cells.
Transformed cells are those cells which have taken up the plasmid DNA. When the cells are grown on antibiotic-containing medium, only transformed cells will survive.
In the image, C, which is ampR along with tetR are the antibiotic resistance genes.
Here, in the image, D is "ori" or origin of replication. This is the region from where replication is initiated.