Identify the correct pair of combinations.
I. 14C - Distinction between PS-I and PS-II.
II. 15N - Semiconservation replication of DNA.
III. 35S - Polypeptide synthesis.
IV. 32C - Identification of chemical nature of genetic material
A
II and III
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B
II and IV
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C
I and II
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D
I and III
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Solution
The correct option is A II and III 15N isotope was used by Meselson and Stahl to experimentally determine the semi-conservative method of DNA replication. Hence, is the correct answer. 14C was used by Dr. Calvin to trace the metabolic fate of carbon during photosynthesis. 35S was used in the Hershey and Chase experiment to prove that the genetic material is DNA. 32P was the isotope used in the Hershey and Chase experiment which proved that DNA and not protein, was the genetic material.