Question

Identify the correct sequence of tools used in genetic engineering.

• A. Restriction enzymes $\to$ Ligase $\to$ Vectors $\to$ Markers
• B. Restriction enzymes $\to$ Vectors $\to$ Ligase $\to$ Markers
• C. Markers $\to$ Restriction enzyme $\to$ Vectors $\to$ Ligase
• D. Vectors $\to$ Restriction enzymes $\to$ Ligase $\to$ Markers

Answer 1

The correct option is B Restriction enzymes $\to$ Vectors $\to$ Ligase $\to$ Markers

Restriction enzymes are used to cut the donor DNA and the plasmid DNA which acts as a vector. Once both are cut at specific sites, the desired gene is ligated to the vector with the help of DNA ligase. The recombinant DNA is then introduced into the host organism. The recombinant organisms are selected by the use of markers which helps us to screen out the non-transformed organisms.