Question

Identify the correct statement w.r.t. restriction enzyme.

• A. Restriction enzymes can cut the strands of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands
• B. Restriction enzymes cut the strands of DNA a little closer towards the centre of the palindrome sites, but between the two different bases on the opposite strands
• C. Sticky ends are named so because they form peptide bonds with their complementary cut counterparts
• D. The stickiness of the ends do not facilitate the action of DNA ligase

Answer 1

The correct option is A Restriction enzymes can cut the strands of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands

Restriction enzymes can cut the strands of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme, DNA ligase.