Question

Identify the desirable characteristics for a plasmid used in rDNA technology from the following.

$A$. Ability to multiply and express outside the host in a bioreactor.
$B$. A highly active promoter.
$C$. A site at which replication can be initiated.
$D$. One or more identifiable marker genes.
$E$. One or more unique restriction sites.

• A. A, C, and E only
• B. B, C, and E only
• C. A, C, D, and E only
• D. B, C, D, and E only

Answer 1

The correct option is D B, C, D, and E only

The plasmid is the small circular DNA. These are extra chromosomal DNA which can replicate individually. The plasmid is inserted with a desired gene and then introduced in the host cell. The characters which makes plasmid suitable to be used in rDNA technology and genetic engineering techniques:

• There should be an active promoter site and the origin of replication site called as Ori site.
• These regions will allow the plasmid to replicate in the host.
• There should be marker genes which can be used to identify the recombinants.
• In order to insert the desired gene, there should be presence of restriction sites.

Thus, the correct answer is option D.