Question

Identify the enzymes catalysing reactions (A) and (B)

(A)$\alpha$-ketoglutarate + ${NH}^{4+}$ + NADPH $\to$ Glutamate + $H_{2}O$ + $NAD{P}^{+}$

(B)$R-CH-\left(COO\right)N{H}_2+R_1-\left(CO\right)COO\to R-\left(CO\right)COO+R_1-CH-\left(COO\right)N{H}_2$

• A. A - Glutamate dehydrogenase, B - Transaminase
• B. A - Transaminase, B - Glutamate dehydrogenase
• C. A - Nitrogenase, B - Transaminase
• D. A - Glutamate aminase, B - Dehydrogenase

Answer 1

The correct option is A

A - Glutamate dehydrogenase, B - Transaminase

In reductive amination, ammonia reacts with $\alpha$-ketoglutaric acid and forms glutamic acid in the presence of glutamate dehydrogenase. Transamination involves the transfer of amino group from an amino acid to keto group of a keto acid in the presence of transaminase.