3
Question
Identify the labels A, B, C, D and E and choose the correct option.
Identify the labels A, B, C, D and E and choose the correct option.
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Solution
The correct option is B A - Vector DNA, B - Foreign DNA, C - EcoR I, D - Joining of DNA fragments, E - Recombinant DNA
The given illustration represents the steps involved in the formation of recombinant DNA by the action of the restriction endonuclease - EcoR I.
DNA molecules that have segments from two different biological sources/organisms are called recombinant DNA. This is possible to be produced because there are enzymes called restriction enzymes that can cut the DNA at specific sites in both organisms and another enzyme called DNA ligase that helps to join the DNA fragments.
A represents vector DNA and B represents foreign DNA (gene of interest).
Vector DNA carries the gene of interest into host cells.
Both vector DNA and foreign DNA contain a recognition site for the restriction enzyme EcoR I.
C represents EcoR I. This enzyme recognizes the following palindromic sequence and cleaves it between G and A.
5' GAATTC 3'
3' CTTAAG 5'
As the restriction enzymes cut both the strands of DNA molecules at different positions, the resulting DNA fragments have short single-stranded overhangs at each end. These are called sticky ends.
The sticky ends in both foreign and vector DNA can be joined using DNA ligase.
D in the image represents the joining of DNA fragments at the sticky ends. This results in the formation of recombinant DNA which is also called chimeric DNA.
E in the image represents the recombinant DNA.
BamH I is a restriction enzyme and the recognition site for BamH I is
5' —— GGATCC —— 3'
3' —— CCTAGG —— 5'
Sir Alec Jeffreys developed a method of DNA fingerprinting. This method involves identifying a person based on certain repetitive sequences in the DNA.
The given illustration represents the steps involved in the formation of recombinant DNA by the action of the restriction endonuclease - EcoR I.
DNA molecules that have segments from two different biological sources/organisms are called recombinant DNA. This is possible to be produced because there are enzymes called restriction enzymes that can cut the DNA at specific sites in both organisms and another enzyme called DNA ligase that helps to join the DNA fragments.
A represents vector DNA and B represents foreign DNA (gene of interest).
Vector DNA carries the gene of interest into host cells.
Both vector DNA and foreign DNA contain a recognition site for the restriction enzyme EcoR I.
C represents EcoR I. This enzyme recognizes the following palindromic sequence and cleaves it between G and A.
5' GAATTC 3'
3' CTTAAG 5'
As the restriction enzymes cut both the strands of DNA molecules at different positions, the resulting DNA fragments have short single-stranded overhangs at each end. These are called sticky ends.
The sticky ends in both foreign and vector DNA can be joined using DNA ligase.
D in the image represents the joining of DNA fragments at the sticky ends. This results in the formation of recombinant DNA which is also called chimeric DNA.
E in the image represents the recombinant DNA.
BamH I is a restriction enzyme and the recognition site for BamH I is
5' —— GGATCC —— 3'
3' —— CCTAGG —— 5'
Sir Alec Jeffreys developed a method of DNA fingerprinting. This method involves identifying a person based on certain repetitive sequences in the DNA.
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