Question

Identify the steps that are involved in PCR.
(A) Denaturation
(B) Annealing
(C) Extension
(D) Down stream processing

• A. B, D, C
• B. A, D, B
• C. A, B, C
• D. A, C, D

Answer 1

The correct option is C A, B, C

• PCR stands for a polymerase chain reaction. This is a technique used to amplify a given sample of DNA.
• In this the first step is to denature the DNA template by melting the hydrogen bonds and making it into a single strand at 94 degrees celsius.
• The second step involves the annealing step that involves joining the primers to the single-stranded DNA.
• Then this step is followed by the extension or elongation step that involves the extension of the DNA by the Taq polymerase by the addition of the nucleotides.

Hence, option C is correct.