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Question
Identify the vector suitable for cloning long DNA fragments :
Identify the vector suitable for cloning long DNA fragments :
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Solution
The correct option is B Cosmids
Answer is option D i.e. "Cosmids"
Two types of vectors are cloning and expression vectors. One of the ideal character of a coning vector is to carry and successfully clone long DNA fragments. Cosmids are used to clone DNA segments upto 50 kb (28kb - 50 kb).
While, phage vector can be used to clone DNA segments upto 10 kb
Bacterial plasmids (BAC) can be used to clone DNA segments upto 20 kb (10kb - 20kb).
Yeast plasmids (YAC) are expression vectors and are not used as cloning vectors. YAC can be used to express DNA segments upto 500 kb (100kb - 500kb).
Answer is option D i.e. "Cosmids"
Two types of vectors are cloning and expression vectors. One of the ideal character of a coning vector is to carry and successfully clone long DNA fragments. Cosmids are used to clone DNA segments upto 50 kb (28kb - 50 kb).
While, phage vector can be used to clone DNA segments upto 10 kb
Bacterial plasmids (BAC) can be used to clone DNA segments upto 20 kb (10kb - 20kb).
Yeast plasmids (YAC) are expression vectors and are not used as cloning vectors. YAC can be used to express DNA segments upto 500 kb (100kb - 500kb).
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