Identify the wrong statement with regard to Restriction Enzymes.
Identify the wrong statement with regard to Restriction Enzymes.
The correct option is B Sticky ends can be joined by using DNA ligases.
The correct option is B:
Explanation of the correct answer:
- Restriction enzymes are part of a larger category of enzymes known as nucleases. Exonucleases and endonucleases are the two types.
- Endonucleases make cuts at specified places within the DNA, whereas exonucleases remove nucleotides off the ends.
- The length of a DNA sequence is 'inspected' by each restriction endonuclease. It will connect to the DNA and cut each of the two strands of the double helix at precise spots in their sugar phosphate backbones after it has found its specific recognition sequence.
- Each restriction endonuclease recognises nucleotide sequences in DNA that are palindromic.
- In genetic engineering, restriction endonucleases are employed to create'recombinant' DNA molecules, which are made up of DNA from different sources/genomes.
Explanation of the incorrect option:
Option A:
In genetic engineering, restriction endonucleases are employed to create'recombinant' DNA molecules, which are made up of DNA from different sources/genomes.
Option C:
The length of a DNA sequence is 'inspected' by each restriction endonuclease. It will connect to the DNA and cut each of the two strands of the double helix at precise spots in their sugar phosphate backbones after it has found its specific recognition sequence.
Option D:
Each restriction endonuclease recognises nucleotide sequences in DNA that are palindromic.
Final answer: Sticky ends have free, dangling, or unpaired nitrogen bases that can link with complementary bases on other DNA segments to form recombinant DNA. In the absence of sticky ends, a ligase is necessary to link two DNA segments together.
Sticky ends can be joined by using DNA ligases.
The correct option is B:
Explanation of the correct answer:
- Restriction enzymes are part of a larger category of enzymes known as nucleases. Exonucleases and endonucleases are the two types.
- Endonucleases make cuts at specified places within the DNA, whereas exonucleases remove nucleotides off the ends.
- The length of a DNA sequence is 'inspected' by each restriction endonuclease. It will connect to the DNA and cut each of the two strands of the double helix at precise spots in their sugar phosphate backbones after it has found its specific recognition sequence.
- Each restriction endonuclease recognises nucleotide sequences in DNA that are palindromic.
- In genetic engineering, restriction endonucleases are employed to create'recombinant' DNA molecules, which are made up of DNA from different sources/genomes.
Explanation of the incorrect option:
Option A:
In genetic engineering, restriction endonucleases are employed to create'recombinant' DNA molecules, which are made up of DNA from different sources/genomes.
Option C:
The length of a DNA sequence is 'inspected' by each restriction endonuclease. It will connect to the DNA and cut each of the two strands of the double helix at precise spots in their sugar phosphate backbones after it has found its specific recognition sequence.
Option D:
Each restriction endonuclease recognises nucleotide sequences in DNA that are palindromic.
Final answer: Sticky ends have free, dangling, or unpaired nitrogen bases that can link with complementary bases on other DNA segments to form recombinant DNA. In the absence of sticky ends, a ligase is necessary to link two DNA segments together.
