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Question
If a part of pith from the stem of a plant is used as an explant and cultured on nutrient medium, which of the following processes is responsible for the formation of an undifferentiated mass of cells called callus?
If a part of pith from the stem of a plant is used as an explant and cultured on nutrient medium, which of the following processes is responsible for the formation of an undifferentiated mass of cells called callus?
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Solution
The correct option is D Dedifferentiation
The portion of pith taken as an explant comprises of parenchymatous cells (i.e., simple permanent tissue which have lost the capacity to divide). When such cells are cultured on solid culture media, the parenchymatous cells of pith become meristematic and start dividing resulting in a mass of undifferentiated cells called callus. This is an example of dedifferentiation.
The portion of pith taken as an explant comprises of parenchymatous cells (i.e., simple permanent tissue which have lost the capacity to divide). When such cells are cultured on solid culture media, the parenchymatous cells of pith become meristematic and start dividing resulting in a mass of undifferentiated cells called callus. This is an example of dedifferentiation.
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