If E.coli with. Heavy DNA is allowed to grow for 80 minutes in a normal NH4Cl medium ratio of Light and heavy DNA is
If E.coli with. Heavy DNA is allowed to grow for 80 minutes in a normal NH4Cl medium ratio of Light and heavy DNA is
E. coli bacteria divide in every 20 min so in 80 min it will be four generations.
N15 isotope is present in the environment than shifted in the N14 environment, so it will result in the presence of N15 and N14 strands in DNA.
In the second generation, half will have the previous hybrid bacteria and half will have the light bacteria.
In the third generation, the hybrid generation will again cut half, this time 25% hybrid and 75% light. Now the ratio of light to heavy is 3:1.i.e., 1/4 will be heavy and 3/4 will be light.
In the fourth generation, the above four bacteria divide to give 8 bacteria. Out of it, 1 will be heavy and 7 will be light. Therefore, the ratio of light to heavy is 1:7.
E. coli bacteria divide in every 20 min so in 80 min it will be four generations.
N15 isotope is present in the environment than shifted in the N14 environment, so it will result in the presence of N15 and N14 strands in DNA.
In the second generation, half will have the previous hybrid bacteria and half will have the light bacteria.
In the third generation, the hybrid generation will again cut half, this time 25% hybrid and 75% light. Now the ratio of light to heavy is 3:1.i.e., 1/4 will be heavy and 3/4 will be light.
In the fourth generation, the above four bacteria divide to give 8 bacteria. Out of it, 1 will be heavy and 7 will be light. Therefore, the ratio of light to heavy is 1:7.
