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Question
If the “annealing” step is missed during PCR, what would be its effect on the entire process?
If the “annealing” step is missed during PCR, what would be its effect on the entire process?
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Solution
The correct options are
B Primers will not attach.
C DNA Polymerase would not work.
D DNA will not extend.
PCR occurs in three steps: - Denaturation: It maintains a temperature at 94°C. The high temperature melts the hydrogen bonds and separates the two strands of DNA.
- Anealing: It occurs at 55°C. The two oligonucleotide primers anneal with the separate strands.
- Extension: It carries out at 72°C. Finally, DNA polymerase froms the complimentary strand.
Hence if the "Annealing" step is missed the primers will not attach. DNA polymerase cannot function without a primer and therefore DNA will not extend.
B Primers will not attach.
C DNA Polymerase would not work.
D DNA will not extend.
PCR occurs in three steps:
- Denaturation: It maintains a temperature at 94°C. The high temperature melts the hydrogen bonds and separates the two strands of DNA.
- Anealing: It occurs at 55°C. The two oligonucleotide primers anneal with the separate strands.
- Extension: It carries out at 72°C. Finally, DNA polymerase froms the complimentary strand.