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Chemical Safety Tests

If the gene o...

Question

If the gene of interest was cloned at BamHI site in pBR322, the recombinant bacteria now would conclusively exhibit resistance to drug:

A

Tetracycline

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B

Ampicillin

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C

Neomycin

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D

Kanamycin

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Solution

The correct option is B Ampicillin
If gene of interest is cloned at BamHI site in pBR322, the recombinant loses its resistance to tetracycline by insertional inactivation. Hence, the recombinant bacteria now would conclusively exhibit resistance to ampicillin.

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