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Question

If you are provided with DNA polymerase from E.coli and from Thermus aquaticus, which one would you choose to perform PCR? Justify. [3 marks]

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Solution

DNA polymerase from Thermus aquaticus would be used to perform PCR. [1 mark]

Thermus acquaticus is a thermophilic bacteria. Enzymes isolated from this bacteria can tolerate high temperatures and remain active. [1 marlk]

In PCR, high temperature of 90-94 degrees is provided for denaturation of DNA. DNA polymerase from Thermus aquaticus will be able to function at this high temperature. [1 mark]

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