Question

imparts a positive charge to the cell, which makes it competent for heat shock treatment.

• A. ${\mathrm{CaCl}}_2$
• B. $\mathrm{Ca}{\left({\mathrm{NO}}_3\right)}_2$
• C. $\mathrm{FCl}$
• D. $\mathrm{HCl}$

Answer 1

The correct option is A ${\mathrm{CaCl}}_2$

The cell membrane has a negative charge. This is not conducive for the transformation process. $CaC{l}_2$ gives the cell an overall positive charge. This helps the cell to accept the negatively charged DNA. Therefore it is an important step in the heat shock treatment.