Question

In a genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Which of the following can be used as a DNA vectors?

• A. Bacteriophage
• B. Plasmid
• C. Plasmodium
• D. Both A and B

Answer 1

The correct option is D Both A and B

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells and they also occur in some eukaryotes. Often, the genes carried in plasmids provide bacteria with genetic advantages, such as antibiotic resistance. Plasmids that are used experimentally for these purposes are called as vectors. The vector is the main participant in cloning. Two types of DNA vectors can replicate in bacteria: plasmids and bacteriophages. A plasmid can reside in a bacterium, independently of its genome. The plasmid, will not destroy the cell. The bacteriophage are viruses that infect bacteria and can multiply in a bacterium, and will lyse the cell at the end of its cycle and release virus particles into the medium.

Thus, the correct answer is option D.