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Question

In a PCR machine, which of the following is used to produce multiple copies of DNA?

A
Agrobacterium
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B
Escherichia coli
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C
Thermus aquaticus
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D
Lactobacillus
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Solution

The correct option is C Thermus aquaticus
  • The thermal cycler is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR).
  • It is also known as a thermocycler, PCR machine or DNA amplifier.
  • Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including but not limited to restriction enzyme digestion or rapid diagnostics.
  • Later, the PCR process was adapted to the use of thermostable DNA polymerase enzyme from Thermus aquaticus, which greatly simplified the design of the thermal cycler.
  • So, the correct answer is 'Thermus aquaticus'

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