In addition to Taq polymerase enzyme which other thermostable DNA polymerases have been isolated to be used in polymerase chain Reaction (PCR)?
A
Pfu polymerase isolated from Pyrococcus furiosus
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B
Tli polymerase (vent polymerase) isolated from Thermococcus litoralis
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C
Both (a) and (b)
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D
None of these
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Solution
The correct option is A Both (a) and (b) In addition to Tag DNA polymerase, Pfu polymerase and Tli polymerase have been isolated which are also thermostable. Pfu polymerase is isolated from Pyrococcus furiosus. Tli (vent) polymerase is isolated from Thermococcus litoralis.