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Question

In agarose gel electrophoresis, the separated DNA fragments can be visualised after staining the DNA with EtBr under:

A
Gamma rays
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B
Infrared light
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C
X-rays
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D
Ultraviolet radiation
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Solution

The correct option is D Ultraviolet radiation
  • The separated DNA fragments can be visualised only after staining the DNA with EtBr under UV radiation.
  • Absorption range for UV rays is between 210-280 nm.
  • Ethidium bromide is an intercalating agent that gets stacked in between nitrogenous bases.

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