Question

In bacterial$/$ tissue culture, glassware and nutrients are sterilized through

• A. Water bath at ${200}^o$C
• B. Dry air oven at ${200}^o$C
• C. Dehumidifier
• D. Autoclave

Answer 1

The correct option is D Autoclave

Sterilization by autoclaving is carried out to kill bacteria and other pathogens present in the nutrients, equipment, and materials. In bacterial/ tissue culture, glassware and nutrients are sterilized through autoclave. In this, a pressurized steam is applied with the heat of 121${}^0$C for around 15–20 minutes to kill bacteria, spores, and other pathogens.

Thus, the correct answer is 'Autoclave.'