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Selection of Transformed Cell

In blue/white...

Question

In blue/white screening, the transformed cells with rDNA will exhibit

A

blue colour

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B

white colour

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C

blue and white colour

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D

no colonies

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Solution

The correct option is B white colour
In blue/white screening, the transformed cells with rDNA will show white colour. The untransformed cells contain the lac Z gene which gives blue colour in medium containing X-gal. Whereas, the transformed cells contain inactive lac-Z gene which gives white colour.

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Similar questions

Q. Read the following statements and select the incorrect ones.

(i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells will grow.

(ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules.

(iii) Downstream processing is one of the steps of rDNA technology.

(iv) Disarmed pathogen vectors are also used in the transfer of rDNA into the host.

Q. How many of the following statement(s) regarding insertional inactivation of lacZ gene are incorrect?

1. lacZ gene remains active in non-recombinant transformed bacteria.
2. X-gal gets hydrolysed and produces a blue product in non-recombinant transformed bacteria.
3. Bacterial colonies which are transformed with recombinant DNA appear white.
4. 𝛃-Galactosidase is produced in recombinant bacteria.
5. Bacterial colonies which are transformed with recombinant DNA appear blue.

Q. Screening markers use __ gene that turns the recombinant cells white in a media that is rich is X-Gal

Q. Regarding Assertion and Reason, choose the correct option.
Assertion[A]: Selection markers give straightforward results as to which cells have been transformed.
Reason[R]: Screening markers need X-gal stain to visualize the result of transformation procedure.

Q. A scientist tried to transform bacteria with a plasmid containing the gene of interest inserted within the lacZ gene. Few of the bacteria took up the plasmid while many didn’t. He plated this mixture of bacteria on a medium containing X-gal. Choose the illustration which correctly represents the results he would have obtained based on blue-white screening.

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