Question

In cloning experiments, cDNA molecules can be obtained from an mRNA copy by

• A. Polymerase chain reaction
• B. Reverse transcriptase
• C. Ribozyme
• D. DNA-RNA hybridization

Answer 1

The correct option is B Reverse transcriptase

Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase. cDNA is often used to clone eukaryotic genes in prokaryotes. cDNA is also produced naturally by retroviruses (such as HIV) for integration into the host's genome.

So, the correct option is 'Reverse transcriptase'.