Question

In Cohen and Boyer's recombinant DNA experiments, restriction endonucleases were used to

• A. Isolate fragments of cloned bacterial plasmids.
• B. Isolate fragments of frog DNA that contained an rRNA gene.
• C. Cleave the bacterial plasmid.
• D. All the above

Answer 1

The correct option is C Cleave the bacterial plasmid.

Restriction endonucleases are enzymes which cleave DNA at specific sites. In Cohen and Boyer’s experiment, restriction endonuclease EcoR1 was used to cut the bacterial plasmids containing tetracycline and kanamycin gene respectively.