In DNA and RNA, nitrogen atom is present in the ring system. Can Kjeldahl method be used for the estimation of nitrogen present in these? Give reasons.
In DNA and RNA, nitrogen atom is present in the ring system. Can Kjeldahl method be used for the estimation of nitrogen present in these? Give reasons.
The nitrogen atoms of organic compounds are converted to ammonia (NH3) and this released ammonia is further titrated in Kjeldahl method of estimation of nitrogen present in organic compounds.
This method is not suitable for compounds containing nitrogen in azo and nitro groups or in rings (quinoline, pyridine, etc.). In these cases, the nitrogen cannot be converted to ammonia by following the Kjeldahl method.
In DNA and RNA, the nitrogen atom is present in the ring system. So, Nitrogen present in DNA and RNA cannot be converted to ammonia by following the Kjeldahl method.
Therefore, the Kjeldahl method cannot be used for the estimation of nitrogen present in DNA and RNA.
The nitrogen atoms of organic compounds are converted to ammonia (NH3) and this released ammonia is further titrated in Kjeldahl method of estimation of nitrogen present in organic compounds.
This method is not suitable for compounds containing nitrogen in azo and nitro groups or in rings (quinoline, pyridine, etc.). In these cases, the nitrogen cannot be converted to ammonia by following the Kjeldahl method.
In DNA and RNA, the nitrogen atom is present in the ring system. So, Nitrogen present in DNA and RNA cannot be converted to ammonia by following the Kjeldahl method.
Therefore, the Kjeldahl method cannot be used for the estimation of nitrogen present in DNA and RNA.
