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In DNA finger printing, analysis is made of
In DNA finger printing, analysis is made of
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Solution
The correct option is C Variable number of tandem repeats
About 3% or so of the human genome has highly repetitive sequences or simple-sequence DNA or simple sequence repeats or satellite DNA or microsatellites which are less than 10 bp long short sequences, repeated in multiples per cell. Satellite DNA, also termed as microsatellite, show relative uniformity within species and great variability between closely related species. Because of their short sequence, they are amenable to PCR analysis; options A and C are incorrect. A variable number tandem repeat (or VNTR) refers to the presence of a short nucleotide sequence as a tandem repeat on many chromosomes; it exhibits variations in length and number of repeats between individuals. VNTR are 10-100 bp long. This DNA polymorphism is revealed during DNA finger printing in creating DNA profile of individuals. For the purpose, restriction enzyme digested DNA samples are sorted by gel electrophoresis followed by southern blotting. Finally, radioactive probes are washed over the nylon surface to allow their joining to any DNA fragments of the same composition; option D is correct. The VNTR, SINES, LINES and few redundant genes are categorized as moderately repetitive DNA which is found throughout the genome; SINES and LINES are interspersed repeats which are not used for DNA finger printing; option B is incorrect.
About 3% or so of the human genome has highly repetitive sequences or simple-sequence DNA or simple sequence repeats or satellite DNA or microsatellites which are less than 10 bp long short sequences, repeated in multiples per cell. Satellite DNA, also termed as microsatellite, show relative uniformity within species and great variability between closely related species. Because of their short sequence, they are amenable to PCR analysis; options A and C are incorrect.
A variable number tandem repeat (or VNTR) refers to the presence of a short nucleotide sequence as a tandem repeat on many chromosomes; it exhibits variations in length and number of repeats between individuals. VNTR are 10-100 bp long. This DNA polymorphism is revealed during DNA finger printing in creating DNA profile of individuals. For the purpose, restriction enzyme digested DNA samples are sorted by gel electrophoresis followed by southern blotting. Finally, radioactive probes are washed over the nylon surface to allow their joining to any DNA fragments of the same composition; option D is correct. The VNTR, SINES, LINES and few redundant genes are categorized as moderately repetitive DNA which is found throughout the genome; SINES and LINES are interspersed repeats which are not used for DNA finger printing; option B is incorrect.
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