Question

In DNA fingerprinting technique, the radioactive intermediates formed during hybridization are .......... molecules.

• A. Double-stranded DNA
• B. Single-stranded DNA
• C. Single-stranded mRNA
• D. Double-stranded rRNA

Answer 1

Answer: (B)

Single-stranded DNA

• DNA fingerprinting is the method of isolating and separating the DNA sequence.
• The DNA is cleaved to form smaller fragments. The digested fragments are separated by electrophoresis based on their charge and size.
• The separated fragments are then blotted onto a nitrocellulose membrane. followed by hybridization with radioactive probes.
• These hybridized fragments of single-stranded DNA are then observed and analyzed by using autoradiography.
  Thus the correct answer is option B.