The correct option is C 30%
If guanine constitutes 20% then the adenine would be 30%. According to Chargaff's rule, in DNA, the proportion of adenine always equals to that of thymine and proportion of guanine always equals to that of cytosine, i.e. A=T and G=C. Thus, in the DNA of an organism, if guanine is 20% cytosine also will be 20%. So, both adenine and thymine together will be 60%, i.e. 30% adenine and 30% thymine.