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In Feulgen technique, Schiff reagent reacts with
In Feulgen technique, Schiff reagent reacts with
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Solution
The correct option is B CHO group of deoxyribose
Feulgen technique is an aldehyde specific reaction based on the formation of a purple-colored compound when aldehydes react with fuchsin-sulfuric acid. Deoxyribonucleic acid gives this reaction after removal of its purine bases by acid hydrolysis. It means for the histochemical detection of deoxyribonucleic acid (DNA) in animal and plant cells, as well as in bacteria. It was proposed in 1924 by the German chemist R. Feulgen. The Feulgen reaction consists of two steps. Initially, acid hydrolysis is performed, usually for 812 min, resulting in the cleavage of the nitrogen bases and formation of aldehyde groups. The preparations are then placed in light yellow Schiff reagent (fuchsine-sulfurous acid), which forms bonds with these groups. The red-violet product formed in this step is evidence of the presence of DNA. The Feulgen reaction can be carried out after the use of any fixative. The Feulgen reaction is used for the quantitative determination of DNA. Various modifications of the reaction exist for determining the location and structure of DNA with the aid of electron microscopes. Here, the reagent reacts with CHO or aldehyde group of DNA and not with phosphate group, nitrogen bases and purine nitrogen bases.
Feulgen technique is an aldehyde specific reaction based on the formation of a purple-colored compound when aldehydes react with fuchsin-sulfuric acid. Deoxyribonucleic acid gives this reaction after removal of its purine bases by acid hydrolysis. It means for the histochemical detection of deoxyribonucleic acid (DNA) in animal and plant cells, as well as in bacteria. It was proposed in 1924 by the German chemist R. Feulgen. The Feulgen reaction consists of two steps. Initially, acid hydrolysis is performed, usually for 812 min, resulting in the cleavage of the nitrogen bases and formation of aldehyde groups. The preparations are then placed in light yellow Schiff reagent (fuchsine-sulfurous acid), which forms bonds with these groups. The red-violet product formed in this step is evidence of the presence of DNA. The Feulgen reaction can be carried out after the use of any fixative. The Feulgen reaction is used for the quantitative determination of DNA. Various modifications of the reaction exist for determining the location and structure of DNA with the aid of electron microscopes. Here, the reagent reacts with CHO or aldehyde group of DNA and not with phosphate group, nitrogen bases and purine nitrogen bases.
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