In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors.
(A) A bacterium
(B) Plasmid
(C) Plasmodium
(D) Bacteriophage

(B) and (D) only

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(A), (B) and (D) only

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(A) only

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(A) and (C) only

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Solution

The correct option is B (B) and (D) only
Plasmids are low molecular weight extrachromosomal DNA that carries origin of replication, restriction sites and selectable markers to confer readily selectable phenotypic traits on host cells. Plasmids require very few genes for their own replication and rest of it can be deleted and foreign sequences can be added to the plasmid which in turn makes them a suitable candidate to serve as a vector for gene transfer. The presence of 1/3rd non-essential genome that can be replaced with foreign DNA and essence of the genome to be 40,000 and 53,000 bp long for its packaging makes bacteriophages ideal vector. Thus, the correct answer is option A.

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Similar questions

Q. In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents in this regard and select the correct option about which one or more of these can be used as a vector/vectors?
(i) Bacterium
(ii) Plasmid
(iii) Plasmodium
(iv) Cosmids

(v) Enzyme

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector.
Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors
(i) Bacterium (ii) Plasmid (iii) Plasmodium (iv) Bacteriophage

Q. In a genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Which of the following can be used as a DNA vectors?

Q. Study the following statements regarding recombinant DNA technology and select the incorrect ones

(i) Taq polymerase extends the primers using the nucleotides provided in the reaction

(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology

(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis

(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium

(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture

(vi) Downstream processing is one of the steps of polymerase chain reaction

Q. Regarding assertion and reason, select the correct option
Assertion: Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops.
Reason: A gene incorporated in the bacterial chromosomal gene gets automatically transferred to the crop with which the bacterium is associated.

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